Sequential Injection Analysis of Butyrylcholinesterase Using Butyrylcholine Ion-Selective Electrode Detector
نویسندگان
چکیده
A new automated analytical method for the determination of Butyrylcholinesterase (BChE) based on sequential injection analysis (SIA) using a butyrylcholine chloride (BuCh) ion-selective electrode (ISE) detector was developed. BuCh is degraded to choline and butyrate by enzymatic activity BChE, resulting in decrease concentration. By detecting this change concentration with BuCh-ISE, BChE can be determined indirectly. In present SIA BuCh-ISE α-cyclodextrin membrane, range 0.015–0.50 units/mL at 2.0 mmol dm−3 reaction time 5.0 min. The detection limit 4.8 × 10−3 units/mL.
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ژورنال
عنوان ژورنال: Electrochemistry
سال: 2022
ISSN: ['2186-2451', '1344-3542']
DOI: https://doi.org/10.5796/electrochemistry.22-00095